Part:BBa_K1486015

IFP[2]-CpxR under pBAD

This part code for IFP2 fused at the N terminal of CpxR. The sequence is under pBAD promoter. On top of this construct, three others BioBricks (IFP1-CpxR, CpxR-IFP2, CpxR-IFP1) are construction intermediates to build our four final constructs, which aim to find out the orientation of CpxR homodimerization (check out our four final constructs: BBa_K1486056, BBa_K1486009, BBa_K1486010, BBa_K1486011)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1535
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  Illegal PstI site found at 1535
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1746
  Illegal BamHI site found at 1144
  Illegal XhoI site found at 1920
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1535
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1535
  Illegal AgeI site found at 979
  Illegal AgeI site found at 2331
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961