Part:BBa_K1486004

flexible linker 2x (GGGGS)

Flexible linker used to fuse two proteins.

Usage and Biology

This linker is part of the group of flexible ligands composed of small, non-polar amino acids such as Gly and polar such as Ser. In this way, they guarantee high mobility and offer flexibility for the functional domains of connection. Studies show that when proline residues are exchanged in place of terminal glycine residues, degrees of independence are lost (Ruiz et al., 2016b). In this way, enzyme activity is decreased, which leads to low catalytic efficiencies. We can conclude that flexible ligands provide a reduced catalytic activity for proteins.
The article “Linkers: a synergistic way for chimeric proteins, 2020”, talks about the constitution and interaction of the flexible linker (Gly-Gly-Gly-Gly-Ser)n, where “n” specifies the sum of the repetitions of the residue. These linkers provide partial domain separation. It is possible to observe that the amount of Gly, Ser and Thr residues results in flexibility that allows the mobility of the functional binding domains.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

chassis	E coli
device_type	linker
direction	forward

Isoelectric Point: 5,52 Molecular Weight: 648,59Da

References

1. Michela S, Baenziger-Tobler N, Wibke L, Sarah B, Julian B, Dragan G. Linker Length Matters, Fynomer-Fc Fusion with an Optimized Linker Displaying Picomolar IL-17A Inhibition Potency. IMMUNOLOGY| VOLUME 289, ISSUE 20, P14392-14398, MAY 2014