Coding

Part:BBa_K1485002

Designed by: Kang Yang   Group: iGEM14_NEAU-Harbin   (2014-09-27)

A kind of GFP for eukaryotes with a linker (GS)x5

This part can express GFP well in Aspergillus niger. So we call it eGFP. We add a linker GSGSGSGSGS after the prefix so that someone who wants to make a fusion protein can ligate their part before our part and two kinds of protein will work as usual.


Usage and Biology

This part is useful as a reporter. It works well as expected.

NEAU-Harbin_EGFP.png

The hypha showed under the visual light and UV (fluorescence microscope)



2014NEAU_result_Figure12.png

The hypha showed under the visual light and UV (confocal microscopy)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 754
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aA kind of GFP for eukaryotes with a linker (GS)x5