Coding

Part:BBa_K1485000

Designed by: Kang Yang   Group: iGEM14_NEAU-Harbin   (2014-09-24)

Optimized AmilCP for Aspergillus niger

AmilCP is a kind of chromoprotein that exhibits strong color when expressed. The part BBa_K592009 works well in prokaryote. However, it may not work well in eukaryotes. To improve the function of the part BBa_K592009, we synthesis this part BBa_K1485000.

This part is codon-optimized for expression in A. niger and express amilCP chromoprotein. Codon optimization results was present here. The CAI-Value of amilCP before codon opimization is 0.265, while the one after codon opimization is 0.987. NeauAmilcpbefore.png NEAUAMILCP.png


Usage and Biology

This part is useful as a reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aOptimized AmilCP for Aspergillus niger