Regulatory

Part:BBa_K1484345

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_PiI1, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of inorganic phosphate (Pi) starvation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1661
    Illegal PstI site found at 906
    Illegal PstI site found at 1381
    Illegal PstI site found at 1486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 906
    Illegal PstI site found at 1381
    Illegal PstI site found at 1486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 151
    Illegal BglII site found at 643
    Illegal XhoI site found at 1192
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1661
    Illegal PstI site found at 906
    Illegal PstI site found at 1381
    Illegal PstI site found at 1486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1661
    Illegal PstI site found at 906
    Illegal PstI site found at 1381
    Illegal PstI site found at 1486
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1346
    Illegal BsaI.rc site found at 1881


[edit]
Categories
//promoter
Parameters
None