Regulatory

Part:BBa_K1484343

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_PiI5, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of inorganic phosphate (Pi) starvation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1069
    Illegal SpeI site found at 1792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
    Illegal BamHI site found at 765
    Illegal XhoI site found at 816
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1792
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None