Regulatory

Part:BBa_K1484342

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_PiI3, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription under conditions of inorganic phosphate (Pi) starvation. We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1753
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1753
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1753
    Illegal BglII site found at 1718
    Illegal BamHI site found at 1745
    Illegal XhoI site found at 1043
    Illegal XhoI site found at 1942
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1753
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1691


[edit]
Categories
//promoter
Parameters
None