Regulatory

Part:BBa_K1484339

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI23, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 601
    Illegal EcoRI site found at 1816
    Illegal XbaI site found at 791
    Illegal XbaI site found at 1906
    Illegal PstI site found at 1525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 601
    Illegal EcoRI site found at 1816
    Illegal PstI site found at 1525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 601
    Illegal EcoRI site found at 1816
    Illegal BamHI site found at 44
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 601
    Illegal EcoRI site found at 1816
    Illegal XbaI site found at 791
    Illegal XbaI site found at 1906
    Illegal PstI site found at 1525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 601
    Illegal EcoRI site found at 1816
    Illegal XbaI site found at 791
    Illegal XbaI site found at 1906
    Illegal PstI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 105
    Illegal SapI.rc site found at 1572


[edit]
Categories
//promoter
Parameters
None