Regulatory

Part:BBa_K1484337

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI21, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 251
    Illegal XhoI site found at 258
    Illegal XhoI site found at 1324
    Illegal XhoI site found at 1948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1351
    Illegal AgeI site found at 1151
    Illegal AgeI site found at 1158
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 227
    Illegal BsaI site found at 234
    Illegal BsaI.rc site found at 803


[edit]
Categories
//promoter
Parameters
None