Regulatory

Part:BBa_K1484336

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI20, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 670
    Illegal EcoRI site found at 832
    Illegal XbaI site found at 49
    Illegal XbaI site found at 1142
    Illegal PstI site found at 79
    Illegal PstI site found at 722
    Illegal PstI site found at 1271
    Illegal PstI site found at 1721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 670
    Illegal EcoRI site found at 832
    Illegal PstI site found at 79
    Illegal PstI site found at 722
    Illegal PstI site found at 1271
    Illegal PstI site found at 1721
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 670
    Illegal EcoRI site found at 832
    Illegal BglII site found at 1961
    Illegal BamHI site found at 880
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 670
    Illegal EcoRI site found at 832
    Illegal XbaI site found at 49
    Illegal XbaI site found at 1142
    Illegal PstI site found at 79
    Illegal PstI site found at 722
    Illegal PstI site found at 1271
    Illegal PstI site found at 1721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 670
    Illegal EcoRI site found at 832
    Illegal XbaI site found at 49
    Illegal XbaI site found at 1142
    Illegal PstI site found at 79
    Illegal PstI site found at 722
    Illegal PstI site found at 1271
    Illegal PstI site found at 1721
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1623
    Illegal BsaI.rc site found at 813
    Illegal SapI site found at 161


[edit]
Categories
//promoter
Parameters
None