Regulatory

Part:BBa_K1484332

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI16, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 186
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 590
    Illegal NheI site found at 606
    Illegal NheI site found at 993
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 186
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 492
    Illegal BsaI.rc site found at 502


[edit]
Categories
//promoter
Parameters
None