Regulatory

Part:BBa_K1484331

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI14, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 683
    Illegal EcoRI site found at 1792
    Illegal SpeI site found at 515
    Illegal PstI site found at 1626
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 683
    Illegal EcoRI site found at 1792
    Illegal SpeI site found at 515
    Illegal PstI site found at 1626
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 683
    Illegal EcoRI site found at 1792
    Illegal XhoI site found at 1503
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 683
    Illegal EcoRI site found at 1792
    Illegal SpeI site found at 515
    Illegal PstI site found at 1626
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 683
    Illegal EcoRI site found at 1792
    Illegal SpeI site found at 515
    Illegal PstI site found at 1626
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None