Regulatory

Part:BBa_K1484330

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI13, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1922
    Illegal XbaI site found at 740
    Illegal XbaI site found at 1544
    Illegal PstI site found at 1168
    Illegal PstI site found at 1225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1922
    Illegal NheI site found at 1380
    Illegal PstI site found at 1168
    Illegal PstI site found at 1225
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1922
    Illegal BglII site found at 679
    Illegal BglII site found at 1916
    Illegal XhoI site found at 1048
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1922
    Illegal XbaI site found at 740
    Illegal XbaI site found at 1544
    Illegal PstI site found at 1168
    Illegal PstI site found at 1225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1922
    Illegal XbaI site found at 740
    Illegal XbaI site found at 1544
    Illegal PstI site found at 1168
    Illegal PstI site found at 1225
    Illegal NgoMIV site found at 715
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1137


[edit]
Categories
//promoter
Parameters
None