Regulatory

Part:BBa_K1484327

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI10, marchantia promoter

This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 49
    Illegal PstI site found at 2011
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 49
    Illegal PstI site found at 2011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 572
    Illegal BglII site found at 1820
    Illegal BamHI site found at 4
    Illegal BamHI site found at 1340
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 49
    Illegal PstI site found at 2011
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 572
    Illegal SpeI site found at 49
    Illegal PstI site found at 2011
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1302


[edit]
Categories
//promoter
Parameters
None