Regulatory

Part:BBa_K1484320

Designed by: Angelina Munabi   Group: iGEM14_Cambridge-JIC   (2014-10-10)

P_NiI2, marchantia promoter


This part is an untested potential promoter for Marchantia polymorpha. It is thought to initiate transcription of a nitrate transporter protein under conditions of limiting nitrate (NO-3). We identified the promoter during a broader search for potential inputs to our genetic circuit, and we think it may be used as such, but testing will definitely be required to confirm this. We submit this part as a foundation for future marchantia work. We were able to physically obtain DNA from a PCR of a marchantia genome.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 286
    Illegal XbaI site found at 304
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1650
    Illegal XhoI site found at 1609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 286
    Illegal XbaI site found at 304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 286
    Illegal XbaI site found at 304
    Illegal NgoMIV site found at 1223
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//promoter
Parameters
None