Part:BBa_K1484115

4-coumarate:coenzyme A ligase from N. tabacum

This enzyme catalyses the condensation of p-hydroxycoumaric acid (often called simply p-coumarate) with Coenzyme A, and represents a priming step for engineering the raspberry ketone pathway into E. coli.

When using E. coli, p-coumaric acid can be added to the medium as a feedstock. Levels up to 9mM were tried by Guy Lewy of the Cambridge-JIC 2014 team with no ill effect on the growth of E. coli.

Obtained originally from the lab of Jules Beekwilder and his PhD student Esmer Jongedijk in Wageningen, The Netherlands.

For reference see Microbial production of natural raspberry ketone (Biotechnol. J. 2007, 2, 1270–1279)

Useful as part of the benzalacetone generator which has been shown to work (BBa_K1484317) Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1116
  Illegal BamHI site found at 1462
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1175