Part:BBa_K1484101

GAL4-VP16 transcription activator CDS, compatible with PlantSyntax (RFC105)

GAL4 transcription activator. Binds to the UAS (upstream activation site) for activation of reporter expression in eukaryotes.

This part has been codon optimized for plant expression.

Usage and Biology

GAL4 is a transcription activator which is active in many eukaryotes. It can be used to amplify sensitive inputs. This particular part contains a VP16 fusion to enable the activator to enter the nucleus in plants.

By using an enhancer trap with GAL4, lines can be produced with very specific expression patterns (for example, expression active in a particular tissue). Further circuits utilising reporters driven by the UAS then inherit expression patterns. For example uses, see:

Brand, A. H.; Perrimon, N. (1993). "Targeted gene expression as a means of altering cell fates and generating dominant phenotypes". Development (Cambridge, England) 118 (2): 401–415. PMID 8223268.

Duffy, J. B. (2002). "GAL4 system in Drosophila: A fly geneticist's Swiss army knife". Genesis 34 (1–2): 1–15. doi:10.1002/gene.10150. PMID 12324939.

Luan, Haojiang, Peabody, Nathan C., Vinson, Charles R., and White, Benjamin H. (2006). "Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression". Neuron (Elsevier) 52 (3): 425–436. doi:10.1016/j.neuron.2006.08.028.

This part is Plant Syntax (RFC105) compatible, and can be used as a CDS part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 226
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1
  Illegal BsaI.rc site found at 695
  Illegal SapI site found at 206