Generator

Part:BBa_K1484100

Designed by: Guy Lewy   Group: iGEM14_Cambridge-JIC   (2014-10-04)


4-hydroxybenzalacetone generator

This part can be transformed into E. coli. When the transformants are incubated with p-coumaric acid and 50mM arabinose, they will metabolise the p-coumaric acid and secrete 4-hydroxybenzalacetone into the growth medium.

4-hydroxybenzalacetone is often referred to simply as benzalacetone.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2350
    Illegal BamHI site found at 2696
    Illegal XhoI site found at 3978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3282
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2409
    Illegal SapI site found at 961


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None