Part:BBa_K1480003

TetR generator (Promoter J23109)

This is a TetR protein generator regulated by the constitutive promoter J23109.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

https://parts.igem.org/Part:BBa_K1140006
https://parts.igem.org/Part:BBa_K1480003
https://parts.igem.org/Part:BBa_K145201
https://parts.igem.org/Part:BBa_K1480004
https://parts.igem.org/Part:BBa_K1480005

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]