Composite
Part:BBa_K1480002
Designed by: Jesus Gilberto Rodriguez Ceja Group: iGEM14_UANL_Mty-Mexico (2014-10-08)
This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.
Usage and Biology
It is a reporter protein that switch off when the repressor TetR binds to ptet.
Results
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 638
Illegal AgeI site found at 750 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/rfp
//classic/reporter
//direction/forward
//classic/reporter
//direction/forward
color | Red |
direction | Forward |
emission | 607 |
excitation | 584 |
function | Reporter |
negative_regulators | regulated by TetR |
protein | mRFP |