Coding

Part:BBa_K1480001

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-08)


Transcription Activator-Like Effector Nuclease (TALEN)

This is a synthesized construction that encodes for a Transcription Activator-Like Effector Nuclease (TALEN). TALEN enzyme is useful when you want to make a double strand break in an specific site of the DNA. This part is only the coding sequence for the TALEN. The target nucleotide sequence of the TALEN is GGAGCCATCTGGCCGGGT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1270
    Illegal NheI site found at 1474
    Illegal NheI site found at 1882
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
    Illegal BamHI site found at 2425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1138
    Illegal BsaI.rc site found at 790
    Illegal SapI.rc site found at 2439


[edit]
Categories
Parameters
None