Composite

Part:BBa_K1480000

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-08)


Promoter + RBS + TALEN + Terminator

This is a synthesized construction that encodes for a Transcription Activator-Like Effector Nuclease (TALEN). The gene is under Bba_J23110 constitutive promoter. TALEN enzyme is useful when you want to make a double strand break in an specific site of the DNA. It can be used with the help of primers to add the target site into your vector and then make a loss of function when adding the TALEN due to the double strand break. It also can be used on eukaryotic models to make genome editing. The target nucleotide sequence of the TALEN is GGAGCCATCTGGCCGGGT

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1334
    Illegal NheI site found at 1538
    Illegal NheI site found at 1946
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3083
    Illegal BamHI site found at 2489
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1202
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 2503


[edit]
Categories
Parameters
None