Part:BBa_K1475009

Limonene synthase with tet promoter, RBS and terminator

This part contains the LIMS1 gene from Citrus limon encoding (R)-limonene synthase ([http://www.uniprot.org/uniprot/Q8L5K3 UniProt:Q8L5K3] [http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20 BRENDA:EC 4.2.3.20]). The gene is under the control of the TetR repressible promoter, the tetR repressor can be found as bacis parts with Part:BBa_C0040, or without Part:K1475003 the LVA rapid degradation tag.

Usage and Biology

The LIMS1 gene encodes the (R)-limonene synthase from Citrus limon. (R)-limonene synthase conversion of geranyl diphosphate into (R)-limonene and diphosphate. [2]
(R)-limonene is the main component of lemon scent and is for instance used in food manufacturing.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 178
  

References

1. UniProt, accession number: Q8L5K3. http://www.uniprot.org/uniprot/Q8L5K3
2. BRENDA, 2014: EC 4.2.3.20 - (R)-limonene synthase. http://www.brenda-enzymes.org/enzyme.php?ecno=4.2.3.20&Suchword=&organism%5B%5D=Citrus+limon&show_tm=0