Device

Part:BBa_K1475007

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU_Denmark   (2014-10-05)

GFP controlled by lacI and plac

This composite part has GFP controlled by a lacI, p(lacI) expression system.
Please note that this part contains the lacI repressor without the LVA rapid degradation tag. The lacI+LVA bacis part can be found here: Part:BBa_C0012.


Usage and Biology

This part can be used to test the expression from the lacI, p(lacI) system.
The operation of lacI repressor is inhibited by allolactose and IPTG (Isopropylthiogalactoside). IPTG is preferred because it cannot be degraded by Escherichia coli and therefore remains at a constant concentration.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2057



References

1. Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). http://www.nature.com/nature/journal/v403/n6767/full/403335a0.html
UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

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