Coding

Part:BBa_K1475002

Designed by: Daniel Weltz Pedersen   Group: iGEM14_SDU-Denmark   (2014-08-22)

FAT-2 delta 12 desaturase from C. elegans

This part contains only the coding region of the fat-2 ∆12-desaturase from “Caenorhabditis elegans” ([http://www.uniprot.org/uniprot/G5EGA5 UniProt:G5EGA5]):, the natural gene consists of three exons [1].


Usage and Biology

Fatty acyl desaturases act on acyl groups to introduce double bonds. Fat-2 is a ∆12 fatty acyl desaturase which is able to introduce a double bond in C16 and C18 fatty acids in Caenorhabditis elegans, and it has been shown to work as a ∆15 desaturase in yeast using oleic acid (C18:1∆9) as a substrate [2]. In nature the desaturation of fatty acids is important for keeping the cell membrane fluid in cold conditions [1].
Desaturases that are able to introduce double bonds beyond carbon 9 and 10 are interesting from a nutritional point of view because mammals lack this ability. This is why ω-6 linoleic acid and ω-3 linolenic acid is classified as essential fatty acids for mammals and these needs to be supplied through the diet.

Other desaturases has been submitted to the registry:

Delta-12 desaturase from Synechocystis sp. PCC 6803
By Mancherster 2013: Part:BBa_K1027002
By St. Andrews 2012: Part:BBa_K925000

Delta-6 desaturase from Synechocystis sp. PCC 6803
By St. Andrews 2012: Part:BBa_K925003

Delta-9 Desaturase from Synechocystis sp.
By Manchester 2013: Part:BBa_K1027001

Delta-15 desaturase from Synechocystis sp. PCC 6803
By St. Andrews 2012: Part:BBa_K925001


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
    Illegal BamHI site found at 253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 169
    Illegal SapI site found at 582


References

1. Wormbase, 10 Jun 2010: fat-2. http://www.wormbase.org/species/c_elegans/gene/WBGene00001394?query=fat-2#0-9e-3.
2. Xue-Rong Zhou, et al.: Caenorhabditis elegans Δ12-Desaturase FAT-2 Is a Bifunctional Desaturase Able to Desaturate a Diverse Range of Fatty Acid Substrates at the Δ12 and Δ15 Positions. J Biol Chem. Dec 23, 2011; 286(51): p. 43644–43650, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3243535/
3. Uniprot, fat-2_CAEEL accession number: G5EGA5. http://www.uniprot.org/uniprot/G5EGA5

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