Part:BBa_K1473000

Description

This part is a phagemid combined with RFP (BBa_J04450). Phagemid pBluescript is a special plasmid, it functions as a normal plasmid when transformed into bacteria. But when helper phage M13KO7 infect this bacteria, it will produce progeny M13 phage as vector for sequence on its MCS. Phagemid pBluescript contains a strong package signal upstream on its MCS while M13KO7 helper phage contains a lower package signal for its genome. So after the infection of M13KO7, its genome can produce protein but these proteins will see pBluescript’s MCS as their true “genome”. As a result, the progeny phage’s genome will only contains pBluescript’s MCS, they cannot make next generation. Here we put a reporter gene --- RFP (BBa_J04450) into our pBluescript, so when we transformed this phagemid into bacteria and it should have red fluorescence. If then we add M13KO7 to infect this bacteria, other bacteria nearby should have red fluorescence, too.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2908
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2914
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2908
  Illegal XhoI site found at 2896
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2908
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2908
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2923
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 2357
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 1380
  Illegal SapI site found at 297