Coding

Part:BBa_K1472610

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-28)

Delta 15 desaturase

This sequence encodes the delta 15 desaturase enzyme. The gene sequence from Synechocystis sp. was obtained from NCBI and also be found in the part: BBa_K925001. The DNA sequence was codon-optimized for the expression in E. coli and the codon-optimized gene was synthesized commercially by Life Technologies (Carlsbad, CA, USA). Delta 15 desaturase converts linoleic acid (18:2) to alpha-linolenic acid (ALA; 18:3) by catalyzing the formation of a cis-double bond between carbon 15 and carbon 16 (Figure 1).

CityU HK newbb de15.png

Figure 1. Conversion of linoleic acid into alpha-linolenic acid by delta 15 desaturase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None