Generator

Part:BBa_K1472608

Designed by: Lau Tsz Kit   Group: iGEM14_CityU_HK   (2014-09-25)


Oleic acid (18:0) -> alpha-Linolenic acid (18:3-9,12,15)

It is a device that contained a promoter (BBa_R0010), RBS (BBa_B0034) for each desaturase gene ∆9,12 and ∆15). When free fatty acids, for example, stearic acid (18:0) enters cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linolenic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-Linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase. This device is designated to express under the presence of IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None