Coding

Part:BBa_K1470007

Designed by: Dennis Zimmer   Group: iGEM14_Freiburg   (2014-10-08)

Cas9-Nickase

Cas9-Nickase


Usage and Biology

The 2013 iGEM Freiburg 2013 biobricked an inactive Cas9 (dCas9), which binds to DNA but cannot cleave it anymore and fuctions as a DNA binding protein [1]. We improved this enzyme by a single nucleotide mutation to a nickase. The Cas9-Nickase is capable to induce single strand breaks in the genome, thus leading to a simple method of gene editing 2].

[1] http://parts.igem.org/Part:BBa_K1150000
[2] Cong, L., Ran, F.A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P.D., Wu, X., Jiang, W., Marraffini, L.A., Zhang, F. (2013). Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339 (6121), 819-23

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None