Reporter

Part:BBa_K1468001

Designed by: Pedro Luis Dorado Morales   Group: iGEM14_Valencia_Biocampus   (2014-10-03)

pJ23104 + gene encoding AsRed2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 444
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter
//chassis/multihost
//chassis/prokaryote
//chassis/prokaryote/ecoli
//classic/reporter
//classic/reporter/constitutive
//function/reporter
//function/reporter/fluorescence
//promoter/anderson
//rbs/prokaryote/constitutive/anderson
//regulation/constitutive
//terminator
Parameters
chassisE.coli strains: DH5-alpha, XL1 Blue, JM109, DH10B, HB101, BL21 (DE3)
controlConstitutive promoter: J23104
emissionλem = 607 nm
excitationλex = 584 nm
outputLight
proteinRed Fluorescent Protein (RFP monomeric)