Composite

Part:BBa_K1463345

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-15)


GvpA and GvpC in J61002 Vector

This part contains GvpA with a strong RBS, B0034, and GvpC with a medium RBS, B0032. Both of which are under a constitutive promoter, J23100. This construct is in J61002.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2927
    Illegal NheI site found at 2963
    Illegal NheI site found at 2986
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


[edit]
Categories
Parameters
None