Composite

Part:BBa_K1463207

Designed by: Beth Greig   Group: iGEM14_Glasgow   (2014-08-15)

GvpA in J61002 vector

This part contains GvpA with a constitutive promoter, J23100, and a strong RBS, B0034, in J61002. This construct was created to allow for the easy insertion of the GvpC biobrick downstream by restriction digest. The plasmid containing this construct would be digested using SpeI and PstI, whereas, the biobrick would be digested with XbaI and PstI to allow the formation of a scar site between the end of GvpA and the RBS of GvpC.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



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