Composite
Part:BBa_K1463207
Designed by: Beth Greig Group: iGEM14_Glasgow (2014-08-15)
GvpA in J61002 vector
This part contains GvpA with a constitutive promoter, J23100, and a strong RBS, B0034, in J61002. This construct was created to allow for the easy insertion of the GvpC biobrick downstream by restriction digest. The plasmid containing this construct would be digested using SpeI and PstI, whereas, the biobrick would be digested with XbaI and PstI to allow the formation of a scar site between the end of GvpA and the RBS of GvpC.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |