Composite

Part:BBa_K1462760

Designed by: zhanru Chen   Group: iGEM14_SCUT   (2014-10-14)

GAL1+GBD+SH3+SH3+PDZ+PDZ+PDZ+PDZ+ADH1

Scaffold protein, without Tom22, is a control group, #GBD: #SH3: #PDZ, 1:2:4. Without location, this part can fix the PRK, RuBisCO, CA to a limited space to increases the efficiency of interaction. In this way, We want to compare the effect of physicochemical property of Outer Membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 615
    Illegal BamHI site found at 648
    Illegal BamHI site found at 820
    Illegal BamHI site found at 1029
    Illegal BamHI site found at 1238
    Illegal BamHI site found at 1561
    Illegal BamHI site found at 1884
    Illegal XhoI site found at 801
    Illegal XhoI site found at 1010
    Illegal XhoI site found at 1219
    Illegal XhoI site found at 1542
    Illegal XhoI site found at 1865
    Illegal XhoI site found at 2188
    Illegal XhoI site found at 2511
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None