Protein_Domain

Part:BBa_K1462501

Designed by: zhanru Chen   Group: iGEM14_SCUT   (2014-10-14)

GBD domian + Linker

The GBD domain, one of our scaffold subunits, is a part of GTPase binding domain in Rattus rattus. Now we do the codon opmitization according to our host cell S.cerevisae. The GBD domain allows the assembly of our targeted protein PRK. And the domain is the first subunit of our scaffold protein.

Figure 1. Simulative structure of the GBD domain which we add the linker behind it.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 60
    Illegal BamHI site found at 93
    Illegal BamHI site found at 265
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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