Composite

Part:BBa_K1462280

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)

Gal1+cox6+Crt+cyc1

This part express the third enzyme ----crotonase. Crt is expressed under control of GAL1 promoter and cyc1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462260, BBa_K1462290 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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