Composite

Part:BBa_K1462200

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-14)

Gal1+cox6+AdhE2+GFP+ADH1

This part is constructed as a control group to BBa_K1462150 to identify AdhE2 has been directed to the mitochondria. The GFP is fused at the C-terminus of the enzyme to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3908


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