Coding

Part:BBa_K1462080

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-14)

TOM22

Tom22 is anchored to the outer membrane by its hydrophobic segment in the middle of the sequence and it can carry the precursor protein into the channel formed by Tom40, the β-barrier protein which precursor protein can cross. Now we utilize the presequence of embed protein Tom22 and try to positioned our scaffold protein on the outer mitochondrial membrane.

Figure 1. As shown in the picture, we construct the scaffold protein fusing with the Tom22 which is the member of the Tom family.Tom family is the protein complex that helps the protein including leading peptide enter into the mitochondria. Now We utilize the embed protein Tom22 to make our scaffold protein (the scaffold protein fuse with the Tom22).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None