Coding

Part:BBa_K1462030

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-14)

Hbd

Hbd from Clostridium acetobutylicum encodes 3-hydroxybutyryl-CoA dehydrogenase that catalyzes the chemical reaction.

(S)-3-hydroxybutanoyl-CoA + NADP+ \rightleftharpoons 3-acetoacetyl-CoA + NADPH + H+

In our n-butanol biosynthesis pathway, 3-hydroxybutyryl-CoA dehydrogenase encoded by Hbd catalyzes the rate-limiting reaction that determines the n- butanol production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 387


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/eukaryote/yeast
Parameters
None