Translational_Unit

Part:BBa_K1461314

Designed by: Shunsuke Sumi   Group: iGEM14_UT-Tokyo   (2014-09-26)


pEpCAM-RBS-GFP-4 miR 142 3p non-bindng site-d.term

This is a GFP generator. This generator is used for characterization of translation inhibition by miR-142-3p on EpCAM promoter in HL60 and cos7 as a control. This contains pEpCAM, RBS, GFP, 4 miR-142-3p non-binding sites and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None