Generator

Part:BBa_K1461218

Designed by: Takefumi Yoshikawa   Group: iGEM14_UT-Tokyo   (2014-09-26)


constitutive cr-GFP generator

This part codes GFP, but the RBS of the transcripted mRNA is covered with cis-repressor (cr) sequence and the GFP coding sequence is not translated. taRNA (BBa_K1461003) recover the RBS and activates translation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 745


[edit]
Categories
Parameters
None