Reporter

Part:BBa_K1459017

Designed by: Grzegorz Ścibisz   Group: iGEM14_Warsaw   (2014-10-17)

pmrC-GFP

This is pmrC promoter from Salmonella enterica, with subsequent GFP. pmrC promoter has also a downstream RBS. This part is one part of PmrA-PmrB detecting system. Upon phosphorylation by PmrB, PmrA binds to pmrC and induces expression of GFP. PmrB gains kinase activities after binding ions by it's extracellular loop (eg. iron or terbium atoms, depending on present binding tag).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 25
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 696


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Categories
Parameters
None