Part:BBa_K1459016

Protein name:PmrB Other names:basS, parB Gene name:basS Source organism for the data:Salmonella enterica subsp. enterica serovar Typhimurium str. strain LT2 / SGSC1412 / ATCC 700720 UniProtKB signature:P36557 Gene sequence RefSeq accession number:NC_003197.1 Protein sequence RefSeq accession number:NP_463157.1 Length:356 aa Molecular mass:40,262 Da Cellular localization:inner plasma membrane< Biological function:Signal transduction via kinase acivities PmrB(LBT) is a engineered PmrB gene, where PmrB is a sensor histidine kinase present in the inner cell membrane of many species of bacteria, including E. coli and S. enterica. With a 30 amino acid periplasmic loop, it is capa ble of binding iron (III) and aluminium ions. The binding event induces a conformational change of the protein, which leads to ATP phosphate-derived autophosphorylation of the C-terminal cytoplasmic domain, followed by transfer of the phosphate group onto th e transcriptional regulator PmrA. As part of our project, the periplasmic iron/alumin ium-binding loop of the PmrB was substituted with a synthetic sequence - a lanthanide-binding ta g, intended to bind lanthanide ions, with terbium in particular. Such a binding event would then indu ce the aforementioned conformation change and phosphorylation of the PmrA, leading it to bind to the PmrC promoter, to allow for expression of the Green Fluorescent Protein - our reporter gene.