Primer

Part:BBa_K1456022

Designed by: Mustafa Yılmaz   Group: iGEM14_ATOMS-Turkiye   (2014-10-22)


Glutathione Peroxidase(GPx1) enzyme reverse primer-2 with restriction site, two stop codon and 6xHis

We use this primer to prepare our Glutathione Peroxidase-1 (GPx-1) enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
    Illegal SpeI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
    Illegal SpeI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
    Illegal SpeI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None