Primer

Part:BBa_K1456021

Designed by: Mustafa Yılmaz   Group: iGEM14_ATOMS-Turkiye   (2014-10-22)


Glutathione Peroxidase-1 (GPx-1) enzyme forward primer-2 with restriction site and kozak sequence

We use this primer to prepare our Glutathione Peroxidase (GPx) enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None