Primer

Part:BBa_K1456012

Designed by: Mustafa Yılmaz   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His

We use this primer to prepare our Human Tissue Plasminogen Activator (tPA) enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 14
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHuman Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His