Primer
Part:BBa_K1456012
Designed by: Mustafa Yılmaz Group: iGEM14_ATOMS-Turkiye (2014-08-13)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His
We use this primer to prepare our Human Tissue Plasminogen Activator (tPA) enzyme from cDNA.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 8
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His |