Primer

Part:BBa_K1456011

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Human Tissue Plasminogen Activator (tPA) enzyme forward primer with restriction site and kozak seque

We use this primer to prepare our Human Tissue Plasminogen Activator (tPA) enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
    Illegal NotI site found at 24
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aHuman Tissue Plasminogen Activator (tPA) enzyme forward primer with restriction site and kozak seque