Primer

Part:BBa_K1456010

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Glutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His

we use this primer to prepare our Glutathione Peroxidase (GPx) enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 14
    Illegal SpeI site found at 22
    Illegal PstI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGlutathione Peroxidase (GPx) enzyme reverse primer with restriction site, two stop codon and 6X His