Primer

Part:BBa_K1456009

Designed by: safa tapan   Group: iGEM14_ATOMS-Turkiye   (2014-08-13)


Glutathione Peroxidase (GPx) enzyme forward primer with restriction site and kozak sequence

we use this primer to prepare our Glutathione Peroxidase(GPx)enzyme from cDNA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
    Illegal NheI site found at 50
    Illegal NotI site found at 24
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
    Illegal BamHI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
    Illegal XbaI site found at 14
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGlutathione Peroxidase (GPx) enzyme forward primer with restriction site and kozak sequence