Composite
QPI-AmilCP

Part:BBa_K1455004

Designed by: Camilo Gómez Garzón   Group: iGEM14_Colombia   (2014-10-07)

QPI (TetR inverter) - AmilCP

AmilCP chromoprotein under expression of QPI inverter (tetR).

Bacteria with this device is constitutively blue due to the non-expression of tetR gene. If this device is assembled with any promoter, the reporter amilCP will be work in the reverse way of that promoter, this means: the cells will be blue if the promoter is induced or if it is constitutive, otherwise, the cells will express no color.

It is important to highlight that the inverter-activity does not work if there is tetracycline (or analogues) in the medium.

Here is an example of an E. coli culture carrying this device (with no upstream promoter).

Fotilloinvamil.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None