Part:BBa_K1453506

New TAL_6

Figure 1. The structure of New TAL.

We design seven new sticky ends which get the least score when judging the similarity.

Our parts can be connected in the right order by golden gate method.

New TAL_left_right (BBa_K1453500) can connect both the first part and the last part, so it has two BsmBI recognition site and it can make two sticky ends when digested by BsmBI.

New TAL 1-6 (BBa_K1453501-BBa_K1453506) is intermediate part to make a whole TALE. The TALE can recognize 14bp DNA sequence:

TTCGATATCAAGCT

Seven sticky ends:

AAAA AGGG GTAC GCTC TTTT TCGA CCCC

If you want to know how we design these ends, please go to see our [http://2014.igem.org/Team:SJTU-BioX-Shanghai/Part3_TAL_Improvement project-Part3 TAL improve].

Usage and Biology

Because our TAL improvement is based on the 2012 Freiburg's project, you can just follow [http://2012.igem.org/Team:Freiburg/Project/Tal 2012 Freiburg's protocol ]to connect whole TALE.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 10